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bifidobacterium longum spp infantis atcc 15697  (ATCC)


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    ATCC bifidobacterium longum spp infantis atcc 15697
    Putative glycoside hydrolase genes abundant in high- and medium-quality MAGs from <t>Bifidobacterium</t> ( A ) and Prevotella ( B ) indicate potential HMO-degrading capabilities across related MAGs. Column annotations include infant sample (Infant), maternal weight status (BMI), maternal secretor status (Secretor), MAG completeness (Completeness), and total GH copies (GHSum). Taxonomy was assigned using GTDB-Tk . MAGs are clustered by Euclidean distances.
    Bifidobacterium Longum Spp Infantis Atcc 15697, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1009 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bifidobacterium+longum+spp+infantis+atcc+15697/Bifidobacterium%3B+Longum%3B+Subsp%2E+Infantis/pmc11768495-63-17-21
    Average 99 stars, based on 1009 article reviews
    bifidobacterium longum spp infantis atcc 15697 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "A Pilot Study Exploring the Relationship Between Milk Composition and Microbial Capacity in Breastfed Infants"

    Article Title: A Pilot Study Exploring the Relationship Between Milk Composition and Microbial Capacity in Breastfed Infants

    Journal: Nutrients

    doi: 10.3390/nu17020338

    Putative glycoside hydrolase genes abundant in high- and medium-quality MAGs from Bifidobacterium ( A ) and Prevotella ( B ) indicate potential HMO-degrading capabilities across related MAGs. Column annotations include infant sample (Infant), maternal weight status (BMI), maternal secretor status (Secretor), MAG completeness (Completeness), and total GH copies (GHSum). Taxonomy was assigned using GTDB-Tk . MAGs are clustered by Euclidean distances.
    Figure Legend Snippet: Putative glycoside hydrolase genes abundant in high- and medium-quality MAGs from Bifidobacterium ( A ) and Prevotella ( B ) indicate potential HMO-degrading capabilities across related MAGs. Column annotations include infant sample (Infant), maternal weight status (BMI), maternal secretor status (Secretor), MAG completeness (Completeness), and total GH copies (GHSum). Taxonomy was assigned using GTDB-Tk . MAGs are clustered by Euclidean distances.

    Techniques Used:

    Related Articles

    Generated:

    Article Title: A Pilot Study Exploring the Relationship Between Milk Composition and Microbial Capacity in Breastfed Infants.
    Article Snippet: .. The standard curve was generated from the near-full-length 16S rRNA gene amplified from the genomic DNA of Bifidobacterium longum spp. infantis ATCC 15697 using the 8F and 1492R primers [42] following established protocols [43]. .. Quantitative PCR (qPCR) using a BioRad CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA) was used to determine the number of 16S rRNA gene copies per ng of DNA extracted.

    Article Title: A Pilot Study Exploring the Relationship Between Milk Composition and Microbial Capacity in Breastfed Infants
    Article Snippet: .. The standard curve was generated from the near-full-length 16S rRNA gene amplified from the genomic DNA of Bifidobacterium longum spp. infantis ATCC 15697 using the 8F and 1492R primers [ ] following established protocols [ ]. .. Quantitative PCR (qPCR) using a BioRad CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA) was used to determine the number of 16S rRNA gene copies per ng of DNA extracted.

    Amplification:

    Article Title: A Pilot Study Exploring the Relationship Between Milk Composition and Microbial Capacity in Breastfed Infants.
    Article Snippet: .. The standard curve was generated from the near-full-length 16S rRNA gene amplified from the genomic DNA of Bifidobacterium longum spp. infantis ATCC 15697 using the 8F and 1492R primers [42] following established protocols [43]. .. Quantitative PCR (qPCR) using a BioRad CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA) was used to determine the number of 16S rRNA gene copies per ng of DNA extracted.

    Article Title: A Pilot Study Exploring the Relationship Between Milk Composition and Microbial Capacity in Breastfed Infants
    Article Snippet: .. The standard curve was generated from the near-full-length 16S rRNA gene amplified from the genomic DNA of Bifidobacterium longum spp. infantis ATCC 15697 using the 8F and 1492R primers [ ] following established protocols [ ]. .. Quantitative PCR (qPCR) using a BioRad CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA) was used to determine the number of 16S rRNA gene copies per ng of DNA extracted.

    Infection:

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection
    Article Snippet: .. Caco2 cells were infected with either Bifidobacterium longum spp. infantis ATCC 15697, Salmonella sv. .. Typhimurium LT2 ATCC 700720, or both organisms simultaneously.

    Bacteria:

    Article Title: Peptides from the Intestinal Tract of Breast Milk-Fed Infants Have Antimicrobial and Bifidogenic Activity
    Article Snippet: Mueller-Hinton broth (MHB) and Bacto Agar were obtained from BD (Franklin Lakes, NJ, USA). .. Stock bacteria ( Staphylococcus aureus ATCC 12600, Escherichia coli BL21 (DE3), Bifidobacterium longum spp. infantis ATCC 15697) were obtained from ATCC (Manassas, VA, USA). .. Candidate peptides were synthesized to ≥98% purity by GenScript (Piscataway, NJ, USA).

    other:

    Article Title: The effect of calcium palmitate on bacteria associated with infant gut microbiota
    Article Snippet: Based on the infant gut microbiota composition (Pannaraj et al., ; Yang et al., ), the following bacterial strains were selected: F. prausnitzii A2‐165 (DSM 17677); F. prausnitzii ATCC 27768; F. prausnitzii HTF‐F (DSM 26943); Bifidobacterium longum spp. longum MMB‐01 (referred to as B. longum ); Bifidobacterium longum spp. infantis ATCC 15697 (referred to as B. infantis ); Bifidobacterium breve MMB‐ENR0374; Bifidobacterium bifidum MMB‐02; Bifidobacterium dentidum ATCC 27678; Bifidobacterium adolescentis NIZO B659; Bacteroides fragilis DSM2151; Bacteroides thetaiotaomicron ATCC 29741; Lactobacillus acidophilus DSM 20079; Ruminococcus gnavus MMB‐ENR0435; Collinsella aerofaciens MMB‐03; and Escherichia coli ATCC 29522.

    Purification:

    Article Title: Bread with whole quinoa flour and bifidobacterial phytases increases dietary mineral intake and bioavailability
    Article Snippet: .. Phytases from Bifidobacterium longum spp. infantis ATCC 15697 and Bifidobacterium pseudocatenulatum ATCC 27919 were overexpressed in Escherichia coli strains carrying the bifidobacterial phytase genes and purified by affinity chromatography (Tamayo-Ramos, Sanz-Penella, Yebra, Monedero, & Haros, 2012). ..

    Affinity Chromatography:

    Article Title: Bread with whole quinoa flour and bifidobacterial phytases increases dietary mineral intake and bioavailability
    Article Snippet: .. Phytases from Bifidobacterium longum spp. infantis ATCC 15697 and Bifidobacterium pseudocatenulatum ATCC 27919 were overexpressed in Escherichia coli strains carrying the bifidobacterial phytase genes and purified by affinity chromatography (Tamayo-Ramos, Sanz-Penella, Yebra, Monedero, & Haros, 2012). ..



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    Putative glycoside hydrolase genes abundant in high- and medium-quality MAGs from <t>Bifidobacterium</t> ( A ) and Prevotella ( B ) indicate potential HMO-degrading capabilities across related MAGs. Column annotations include infant sample (Infant), maternal weight status (BMI), maternal secretor status (Secretor), MAG completeness (Completeness), and total GH copies (GHSum). Taxonomy was assigned using GTDB-Tk . MAGs are clustered by Euclidean distances.
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    Adhesion to and invasion of Caco2 cells by S. Typhimurium. (A) Efficacy of different strains t block Salmonella host association. Treatments with “*” are significantly different ( p ≤ 0.5) as compared to control (Caco2 cells incubated with Salmonella alone ). Error bars indicate standard error from 4 replicates. (B) Effect of presence of B. <t>infantis</t> on the relative adhesion and invasion of S. Typhimurium to Caco2 cells. Control (Caco2 cells incubated with S. Typhimurium); Sal-Pre + Bif (Caco2 cells pre-incubated for 30 min with S. Typhimurium and then with B. infantis for 30 more minutes); Bif-Pre+ Sal (Caco2 cells pre-incubated with B. infantis for 30 minutes and then with S. Typhimurium for 60 more minutes); Sal+Bif (Caco2 cells incubated with S. Typhimurium and B. infantis together for 60 minutes); Sal+Bif Incubated ( S. Typhimurium and B. infantis were first incubated together for 60 minutes and then incubated with Caco2 cells for 120 minutes). Treatments that do not share an alphabet are significantly different (p ≤ 0.05). The % numbers of the x axis represents the fraction of total host associated bacteria that were intracellular (invaded). Error bard indicate standard error from three replicates.
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    Image Search Results


    Putative glycoside hydrolase genes abundant in high- and medium-quality MAGs from Bifidobacterium ( A ) and Prevotella ( B ) indicate potential HMO-degrading capabilities across related MAGs. Column annotations include infant sample (Infant), maternal weight status (BMI), maternal secretor status (Secretor), MAG completeness (Completeness), and total GH copies (GHSum). Taxonomy was assigned using GTDB-Tk . MAGs are clustered by Euclidean distances.

    Journal: Nutrients

    Article Title: A Pilot Study Exploring the Relationship Between Milk Composition and Microbial Capacity in Breastfed Infants

    doi: 10.3390/nu17020338

    Figure Lengend Snippet: Putative glycoside hydrolase genes abundant in high- and medium-quality MAGs from Bifidobacterium ( A ) and Prevotella ( B ) indicate potential HMO-degrading capabilities across related MAGs. Column annotations include infant sample (Infant), maternal weight status (BMI), maternal secretor status (Secretor), MAG completeness (Completeness), and total GH copies (GHSum). Taxonomy was assigned using GTDB-Tk . MAGs are clustered by Euclidean distances.

    Article Snippet: The standard curve was generated from the near-full-length 16S rRNA gene amplified from the genomic DNA of Bifidobacterium longum spp. infantis ATCC 15697 using the 8F and 1492R primers [ ] following established protocols [ ].

    Techniques:

    Adhesion to and invasion of Caco2 cells by S. Typhimurium. (A) Efficacy of different strains t block Salmonella host association. Treatments with “*” are significantly different ( p ≤ 0.5) as compared to control (Caco2 cells incubated with Salmonella alone ). Error bars indicate standard error from 4 replicates. (B) Effect of presence of B. infantis on the relative adhesion and invasion of S. Typhimurium to Caco2 cells. Control (Caco2 cells incubated with S. Typhimurium); Sal-Pre + Bif (Caco2 cells pre-incubated for 30 min with S. Typhimurium and then with B. infantis for 30 more minutes); Bif-Pre+ Sal (Caco2 cells pre-incubated with B. infantis for 30 minutes and then with S. Typhimurium for 60 more minutes); Sal+Bif (Caco2 cells incubated with S. Typhimurium and B. infantis together for 60 minutes); Sal+Bif Incubated ( S. Typhimurium and B. infantis were first incubated together for 60 minutes and then incubated with Caco2 cells for 120 minutes). Treatments that do not share an alphabet are significantly different (p ≤ 0.05). The % numbers of the x axis represents the fraction of total host associated bacteria that were intracellular (invaded). Error bard indicate standard error from three replicates.

    Journal: Frontiers in Microbiology

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection

    doi: 10.3389/fmicb.2024.1387498

    Figure Lengend Snippet: Adhesion to and invasion of Caco2 cells by S. Typhimurium. (A) Efficacy of different strains t block Salmonella host association. Treatments with “*” are significantly different ( p ≤ 0.5) as compared to control (Caco2 cells incubated with Salmonella alone ). Error bars indicate standard error from 4 replicates. (B) Effect of presence of B. infantis on the relative adhesion and invasion of S. Typhimurium to Caco2 cells. Control (Caco2 cells incubated with S. Typhimurium); Sal-Pre + Bif (Caco2 cells pre-incubated for 30 min with S. Typhimurium and then with B. infantis for 30 more minutes); Bif-Pre+ Sal (Caco2 cells pre-incubated with B. infantis for 30 minutes and then with S. Typhimurium for 60 more minutes); Sal+Bif (Caco2 cells incubated with S. Typhimurium and B. infantis together for 60 minutes); Sal+Bif Incubated ( S. Typhimurium and B. infantis were first incubated together for 60 minutes and then incubated with Caco2 cells for 120 minutes). Treatments that do not share an alphabet are significantly different (p ≤ 0.05). The % numbers of the x axis represents the fraction of total host associated bacteria that were intracellular (invaded). Error bard indicate standard error from three replicates.

    Article Snippet: Five different probiotic organisms, Bifidobacterium longum spp. infantis (ATCC 15697) , Lactobacillus acidophilus (NCFM) , Lactocaseibacillus casei (ATCC 334) , Lactobacillus gasseri (ATCC 33323), and Levilactobacillus brevis (ATCC 367), were tested for their pathogen exclusion potential during infection with S. Typhimurium using differentiated colonic epithelial cells (Caco2) ( ).

    Techniques: Blocking Assay, Control, Incubation, Bacteria

    Top ten differentially regulated (adj- p ≤ 0) transcriptional regulons in Caco2 cells when exposed to B. infantis .

    Journal: Frontiers in Microbiology

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection

    doi: 10.3389/fmicb.2024.1387498

    Figure Lengend Snippet: Top ten differentially regulated (adj- p ≤ 0) transcriptional regulons in Caco2 cells when exposed to B. infantis .

    Article Snippet: Five different probiotic organisms, Bifidobacterium longum spp. infantis (ATCC 15697) , Lactobacillus acidophilus (NCFM) , Lactocaseibacillus casei (ATCC 334) , Lactobacillus gasseri (ATCC 33323), and Levilactobacillus brevis (ATCC 367), were tested for their pathogen exclusion potential during infection with S. Typhimurium using differentiated colonic epithelial cells (Caco2) ( ).

    Techniques: Binding Assay, Phospho-proteomics, Control, Activity Assay

    Log2 ratios of differentially expressed genes (adj- p ≤ 0.1); caspase 8, 9, 3/7 activity and phosphorylation status of Akt in epithelial cells when they are exposed to B. infantis . For gene expression data, red represents induction while blue represents repression of genes when epithelial cells were exposed to B. infantis .

    Journal: Frontiers in Microbiology

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection

    doi: 10.3389/fmicb.2024.1387498

    Figure Lengend Snippet: Log2 ratios of differentially expressed genes (adj- p ≤ 0.1); caspase 8, 9, 3/7 activity and phosphorylation status of Akt in epithelial cells when they are exposed to B. infantis . For gene expression data, red represents induction while blue represents repression of genes when epithelial cells were exposed to B. infantis .

    Article Snippet: Five different probiotic organisms, Bifidobacterium longum spp. infantis (ATCC 15697) , Lactobacillus acidophilus (NCFM) , Lactocaseibacillus casei (ATCC 334) , Lactobacillus gasseri (ATCC 33323), and Levilactobacillus brevis (ATCC 367), were tested for their pathogen exclusion potential during infection with S. Typhimurium using differentiated colonic epithelial cells (Caco2) ( ).

    Techniques: Activity Assay, Phospho-proteomics, Gene Expression

    (A) Dendogram visualizing similarity between global gene expression profiles of Cac02 cells. The number at each cluster edge represents Approximately Unbiased % p value estimated by multiscale bootstrap resampling 1,000 times. (Host=Cac02 cells incubated with no microbes, Host+Bif=Cac02 cells incubated with B. infantis , Host+Sal=Cac02 cells incubated with S. Typhimurium, Host+Bif+Sal=Cac02 cells simultaneously incubated with 1:1 ratio of B. infantis and S. Typhimurium. The time in minutes represent the time after infection at which the gene expression was determined. (B) Dendogram constructed by hierarchical clustering of small metabolite profile of all the host microbe interaction samples. The number at each cluster edge represents Approximately Unbiased % p value estimated by multiscale bootstrap resampling 1,000 times (Host = Cac02, Bif = B. infantis , Sal = S. Typhimurium). The time in minutes represent the time after infection at which the metabolite profile was determined. “E” represents the extracellular metabolite profile from culture supernatant, while “I” represent the intracellular metabolite profile of all the cells in co-culture.

    Journal: Frontiers in Microbiology

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection

    doi: 10.3389/fmicb.2024.1387498

    Figure Lengend Snippet: (A) Dendogram visualizing similarity between global gene expression profiles of Cac02 cells. The number at each cluster edge represents Approximately Unbiased % p value estimated by multiscale bootstrap resampling 1,000 times. (Host=Cac02 cells incubated with no microbes, Host+Bif=Cac02 cells incubated with B. infantis , Host+Sal=Cac02 cells incubated with S. Typhimurium, Host+Bif+Sal=Cac02 cells simultaneously incubated with 1:1 ratio of B. infantis and S. Typhimurium. The time in minutes represent the time after infection at which the gene expression was determined. (B) Dendogram constructed by hierarchical clustering of small metabolite profile of all the host microbe interaction samples. The number at each cluster edge represents Approximately Unbiased % p value estimated by multiscale bootstrap resampling 1,000 times (Host = Cac02, Bif = B. infantis , Sal = S. Typhimurium). The time in minutes represent the time after infection at which the metabolite profile was determined. “E” represents the extracellular metabolite profile from culture supernatant, while “I” represent the intracellular metabolite profile of all the cells in co-culture.

    Article Snippet: Five different probiotic organisms, Bifidobacterium longum spp. infantis (ATCC 15697) , Lactobacillus acidophilus (NCFM) , Lactocaseibacillus casei (ATCC 334) , Lactobacillus gasseri (ATCC 33323), and Levilactobacillus brevis (ATCC 367), were tested for their pathogen exclusion potential during infection with S. Typhimurium using differentiated colonic epithelial cells (Caco2) ( ).

    Techniques: Gene Expression, Incubation, Infection, Construct, Co-Culture Assay

    GO gene sets that were differentially regulated (adj- p ≤ 0.21) in Caco2 cells when exposed to B. infantis .

    Journal: Frontiers in Microbiology

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection

    doi: 10.3389/fmicb.2024.1387498

    Figure Lengend Snippet: GO gene sets that were differentially regulated (adj- p ≤ 0.21) in Caco2 cells when exposed to B. infantis .

    Article Snippet: Five different probiotic organisms, Bifidobacterium longum spp. infantis (ATCC 15697) , Lactobacillus acidophilus (NCFM) , Lactocaseibacillus casei (ATCC 334) , Lactobacillus gasseri (ATCC 33323), and Levilactobacillus brevis (ATCC 367), were tested for their pathogen exclusion potential during infection with S. Typhimurium using differentiated colonic epithelial cells (Caco2) ( ).

    Techniques: Coagulation, Cell Differentiation, Clinical Proteomics, Membrane, Activity Assay, Antioxidant Activity Assay, Binding Assay, Transferring

    Log2 ratios of gene expression intensities of S. Typhimurium and selected small metabolite peak areas when Cac02 cells were treated with either S. Typhimurium alone or with a co-culture of S. Typhimurium and B. infantis . Induction of genes represent that the gene was induced in Salmonella in presence of B. infantis as compared to its absence.

    Journal: Frontiers in Microbiology

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection

    doi: 10.3389/fmicb.2024.1387498

    Figure Lengend Snippet: Log2 ratios of gene expression intensities of S. Typhimurium and selected small metabolite peak areas when Cac02 cells were treated with either S. Typhimurium alone or with a co-culture of S. Typhimurium and B. infantis . Induction of genes represent that the gene was induced in Salmonella in presence of B. infantis as compared to its absence.

    Article Snippet: Five different probiotic organisms, Bifidobacterium longum spp. infantis (ATCC 15697) , Lactobacillus acidophilus (NCFM) , Lactocaseibacillus casei (ATCC 334) , Lactobacillus gasseri (ATCC 33323), and Levilactobacillus brevis (ATCC 367), were tested for their pathogen exclusion potential during infection with S. Typhimurium using differentiated colonic epithelial cells (Caco2) ( ).

    Techniques: Gene Expression, Co-Culture Assay

    Differentially regulated gene sets (adj- p ≤ 0.09) in S. Typhimurium when incubated with epithelial cells as compared to when incubated with epithelial cells and B. infantis for 120 min.

    Journal: Frontiers in Microbiology

    Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection

    doi: 10.3389/fmicb.2024.1387498

    Figure Lengend Snippet: Differentially regulated gene sets (adj- p ≤ 0.09) in S. Typhimurium when incubated with epithelial cells as compared to when incubated with epithelial cells and B. infantis for 120 min.

    Article Snippet: Five different probiotic organisms, Bifidobacterium longum spp. infantis (ATCC 15697) , Lactobacillus acidophilus (NCFM) , Lactocaseibacillus casei (ATCC 334) , Lactobacillus gasseri (ATCC 33323), and Levilactobacillus brevis (ATCC 367), were tested for their pathogen exclusion potential during infection with S. Typhimurium using differentiated colonic epithelial cells (Caco2) ( ).

    Techniques: Incubation, Glycoproteomics, Membrane, Modification, Transformation Assay